Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods Thomas Kïhler Author

In this laboratory cook-book, the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based prools. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the prools, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR prools into their own clinical or biomedical research.